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They will lose the game _______ they try their best.
A．unlessB．onceC．sinceD．after
A

試題分析：句意:除非他們盡他們的最大努力，否則他們會輸掉比賽。unless 除非，如果不；once 一旦，曾經，一次；since自從；after 在…之后。根據句意可知選A。
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